Tripeptide-29

Key Benefits#

• Stimulates fibroblast collagen synthesis via damage-signal mechanism
• Reduces fine lines and improves skin smoothness
• Supports dermal matrix integrity
• Naturally bioidentical to collagen fragment sequences
• Well-tolerated in all skin types
• Synergistic with copper peptides and retinoids

Mechanism of Action#

As a collagen fragment signal peptide, Tripeptide-29 (Pro-Hyp-Gly) is recognized by fibroblast surface receptors as a collagen breakdown product. This activates COL1A1 and COL3A1 transcription via integrin and discoidin domain receptor (DDR) pathways, increasing procollagen synthesis.

Dosing Protocols#

Side Effects#

• Excellent tolerability profile
• No documented significant adverse effects at cosmetic concentrations
• Rare sensitivity reactions in individuals with peptide allergies

Contraindications#

Known hypersensitivity to collagen-derived peptides

Storage#

Store at room temperature in sealed container away from direct sunlight. Stable across a wide pH range. No special refrigeration required.

1. 1.
   Untargeted LC-MS/MS profiling and semi-quantification of Gly-Pro-Yaa and Gly-Hyp-Yaa tripeptides in collagen hydrolysates

   Bai M, Ma M, Meng Z, Yu J, Zhang M, She W et al. · Talanta · 2025PubMed Verified

2. 2.
   Carbohydrate Co-Solutes Stabilize Collagen Triple Helices

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3. 3.
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4. 4.
   Association between N-Terminal Pyrenes Stabilizes the Collagen Triple Helix

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5. 5.
   Identification, Screening, and Comprehensive Evaluation of Novel DPP-IV Inhibitory Peptides from the Tilapia Skin Gelatin Hydrolysate Produced Using Ginger Protease

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6. 6.
   A solution structure analysis reveals a bent collagen triple helix in the complement activation recognition molecule mannan-binding lectin

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7. 7.
   Evaluating the Properties of Ginger Protease-Degraded Collagen Hydrolysate and Identifying the Cleavage Site of Ginger Protease by Using an Integrated Strategy and LC-MS Technology

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8. 8.
   Oriented Crystallization of Hydroxyapatite in Self-Assembled Peptide Fibrils as a Bonelike Material

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9. 9.
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   Larder CE, Iskandar MM, Kubow S · Current issues in molecular biology · 2021PubMed Verified

10. 10.
    Insertion of Pro-Hyp-Gly provides 2 kcal mol(-1) stability but attenuates the specific assembly of ABC heterotrimeric collagen triple helices

    Wang WM, Yu CH, Chang JY, Chen TH, Chen YC, Sun YT et al. · Organic & biomolecular chemistry · 2021PubMed Verified

11. 11.
    Novel Hyp-Gly-containing antiplatelet peptides from collagen hydrolysate after simulated gastrointestinal digestion and intestinal absorption

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12. 12.
    Decellularization of submillimeter-diameter vascular scaffolds using peracetic acid

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13. 13.
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14. 14.
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15. 15.
    2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks

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16. 16.
    Accelerated endothelialization and suppressed thrombus formation of acellular vascular grafts by modifying with neointima-inducing peptide: A time-dependent analysis of graft patency in rat-abdominal transplantation model

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17. 17.
    Optical control of cell differentiation on synthetic collagen-like scaffolds

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18. 18.
    Collagen Peptide Presenting Nanofibrous Scaffold for Intervertebral Disc Regeneration

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19. 19.
    Amount of Collagen in the Meat Contained in Japanese Daily Dishes and the Collagen Peptide Content in Human Blood after Ingestion of Cooked Fish Meat

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20. 20.
    Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from Clostridium histolyticum Collagenase ColG Increases Bone Formation

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21. 21.
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22. 22.
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23. 23.
    Changes in composition and content of food-derived peptide in human blood after daily ingestion of collagen hydrolysate for 4 weeks

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24. 24.
    Calcium deposition in photocrosslinked poly(Pro-Hyp-Gly) hydrogels encapsulated rat bone marrow stromal cells

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25. 25.
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26. 26.
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27. 27.
    Cytocompatible polyion complex gel of poly(Pro-Hyp-Gly) for simultaneous rat bone marrow stromal cell encapsulation

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28. 28.
    Near-Infrared-Responsive Peptide that Targets Collagen Fibrils to Induce Cytotoxicity

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29. 29.
    Molecular mechanics of the cooperative adsorption of a Pro-Hyp-Gly tripeptide on a hydroxylated rutile TiO2(110) surface mediated by calcium ions

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30. 30.
    Radiolabeled B9958 Derivatives for Imaging Bradykinin B1 Receptor Expression with Positron Emission Tomography: Effect of the Radiolabel-Chelator Complex on Biodistribution and Tumor Uptake

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31. 31.
    Acceleration of bone formation during fracture healing by poly(pro-hyp-gly)10 and basic fibroblast growth factor containing polycystic kidney disease and collagen-binding domains from Clostridium histolyticum collagenase

    Sekiguchi H, Uchida K, Inoue G, Matsushita O, Saito W, Aikawa J et al. · Journal of biomedical materials research. Part A · 2016PubMed Verified

32. 32.
    Fecal Excretion of Orally Administered Collagen-Like Peptides in Rats: Contribution of the Triple-Helical Conformation to Their Stability

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33. 33.
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34. 34.
    Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats

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35. 35.
    Imaging Bradykinin B1 Receptor with 68Ga-Labeled [des-Arg10]Kallidin Derivatives: Effect of the Linker on Biodistribution and Tumor Uptake

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36. 36.
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37. 37.
    Encapsulation of rat bone marrow stromal cells using a poly-ion complex gel of chitosan and succinylated poly(Pro-Hyp-Gly)

    Kusumastuti Y, Shibasaki Y, Hirohara S, Kobayashi M, Terada K, Ando T et al. · Journal of tissue engineering and regenerative medicine · 2017PubMed Verified

38. 38.
    Highly accurate quantification of hydroxyproline-containing peptides in blood using a protease digest of stable isotope-labeled collagen

    Taga Y, Kusubata M, Ogawa-Goto K, Hattori S · Journal of agricultural and food chemistry · 2014PubMed Verified

39. 39.
    Preferred side-chain conformation of arginine residues in a triple-helical structure

    Okuyama K, Haga M, Noguchi K, Tanaka T · Biopolymers · 2014PubMed Verified

40. 40.
    Switchable binding affinity of mannose tethered to collagen peptide by temperature-dependent triple-helix formation

    Okada T, Isobe C, Wada T, Ezaki S, Minoura N · Bioconjugate chemistry · 2013PubMed Verified

41. 41.
    Potential of collagen-like triple helical peptides as drug carriers: Their in vivo distribution, metabolism, and excretion profiles in rodents

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42. 42.
    4(R/S)-Amino/guanidino-substituted proline peptides: design, synthesis and DNA transfection properties

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43. 43.
    Silica precipitation by synthetic minicollagens

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44. 44.
    A peptide study of the relationship between the collagen triple-helix and amyloid

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45. 45.
    Controlling the morphology of metal-promoted higher ordered assemblies of collagen peptides with varied core lengths

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46. 46.
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47. 47.
    The triple helical structure and stability of collagen model peptide with 4(S)-hydroxyprolyl-Pro-Gly units

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48. 48.
    Lysine proximity significantly affects glycation of lysine-containing collagen model peptides

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49. 49.
    Contributions of cation-π interactions to the collagen triple helix stability

    Chen CC, Hsu W, Hwang KC, Hwu JR, Lin CC, Horng JC · Archives of biochemistry and biophysics · 2011PubMed Verified

50. 50.
    Collagen-like polypeptide poly(Pro-Hyp-Gly) conjugated with Gly-Arg-Gly-Asp-Ser and Pro-His-Ser-Arg-Asn peptides enchances cell adhesion, migration, and stratification

    Shibasaki Y, Hirohara S, Terada K, Ando T, Tanihara M · Biopolymers · 2011PubMed Verified