New — Free Peptide Starter Guide (2026): 13 chapters, 34 cited studies

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Ovagen

Also known as: Glu-Asp-Leu, Khavinson liver bioregulator, Hepatic tripeptide

Ovagen is a tripeptide bioregulator (Glu-Asp-Leu) developed by Professor Vladimir Khavinson, primarily targeting liver tissue. It supports hepatocyte function, liver cell regeneration, and protection against hepatic aging and disease. Ovagen is used in protocols for chronic liver disease, hepatoprotection, and metabolic liver conditions including fatty liver disease.

Half-Life

Short (minutes); sustained gene-regulatory effects

Route

SubQ, Oral

Category

Anti-Aging & Longevity

Studies

34 references

Key Benefits

  • Hepatoprotective effects against toxic, viral, and metabolic liver damage
  • Promotes hepatocyte regeneration and liver tissue repair
  • May reduce liver fibrosis progression
  • Supports liver metabolic function and detoxification capacity
  • Anti-aging effects on hepatic tissue
  • Useful in NAFLD/MASH supportive protocols
  • Compatible with NAD+, glutathione, and BPC-157 in liver health stacks

Mechanism of Action

Ovagen's tripeptide sequence Glu-Asp-Leu modulates gene expression in hepatocytes, normalizing protein synthesis essential for liver metabolic function. It reduces hepatocyte apoptosis, supports regenerative capacity of the liver, and may downregulate pro-inflammatory pathways contributing to fibrosis. The peptide acts as a short epigenetic regulator, interacting with chromatin to restore youthful patterns of gene expression in aged or damaged liver tissue.

Dosing Protocols

Standard Khavinson Protocol

Dose
10 mg per day
Frequency
Daily for 10–30 days
Timing
Subcutaneous injection or oral (enteric-coated)
Cycle
10–30 day course, repeated 2–4x per year

Often combined with Livagen and NAD+ in liver health protocols. Research use only.

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Side Effects

  • Generally well tolerated
  • Mild injection site reactions
  • No clinically significant hepatotoxicity reported

Contraindications

Not FDA approved. Not a substitute for antiviral hepatitis therapy, statins, or standard hepatology treatments. Medical oversight recommended for active liver disease.

Storage

Store at 2–8°C. Protect from moisture and light.

  1. 1.
    A Novel Method to Sterilize Isolators for the Housing of Germ-Free Birds and Production of Germ-Free Eggs

    Murphy M, Jones R, Dos Santos A, Adeyemi M, Caulfield C · Applied biosafety : journal of the American Biological Safety Association · 2025PubMed Verified

  2. 2.
    Towards better process management in wastewater treatment plants: Process analytics based on SHAP values for tree-based machine learning methods

    Wang D, Thunéll S, Lindberg U, Jiang L, Trygg J, Tysklind M · Journal of environmental management · 2022PubMed Verified

  3. 3.
    Pulmonary Recruitment Maneuver Reduces Shoulder Pain and Nausea After Laparoscopic Cholecystectomy: A Randomized Controlled Trial

    Kihlstedt Pasquier E, Andersson E · World journal of surgery · 2021RCTPubMed Verified

  4. 4.
    A machine learning framework to improve effluent quality control in wastewater treatment plants

    Wang D, Thunéll S, Lindberg U, Jiang L, Trygg J, Tysklind M et al. · The Science of the total environment · 2021PubMed Verified

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  6. 6.
    Prepared for Mission? A Survey of Medical Personnel Training Needs Within the International Committee of the Red Cross

    Haverkamp FJC, Veen H, Hoencamp R, Muhrbeck M, von Schreeb J, Wladis A et al. · World journal of surgery · 2018PubMed Verified

  7. 7.
    Deaths caused by injury among people of working age (18-64) are decreasing, while those among older people (64+) are increasing

    Bäckström D, Larsen R, Steinvall I, Fredrikson M, Gedeborg R, Sjöberg F · European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2018PubMed Verified

  8. 8.
    Picture book support for preparing children ahead of and during day surgery

    Nilsson E, Svensson G, Frisman GH · Nursing children and young people · 2016PubMed Verified

  9. 9.
    Diastolic orthostatic hypertension and cardiovascular prognosis in type 2 diabetes: a prospective cohort study

    Wijkman M, Länne T, Östgren CJ, Nystrom FH · Cardiovascular diabetology · 2016PubMed Verified

  10. 10.
    Validation-verification of a highly effective, practical human testicular tissue in vitro culture-cryopreservation procedure aimed to optimize pre-freeze and post-thaw motility

    Schiewe MC, Rothman C, Spitz A, Werthman PE, Zeitlin SI, Anderson RE · Journal of assisted reproduction and genetics · 2016PubMed Verified

  11. 11.
    Validation of microSecure vitrification (μS-VTF) for the effective cryopreservation of human embryos and oocytes

    Schiewe MC, Zozula S, Anderson RE, Fahy GM · Cryobiology · 2015PubMed Verified

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  14. 14.
    Transvaginal follicular aspiration and embryo development in superstimulated early postpartum beef cows and subsequent fertility after artificial insemination

    Aller JF, Mucci NC, Kaiser GG, Ríos G, Callejas SS, Alberio RH · Animal reproduction science · 2010RCTPubMed Verified

  15. 15.
    Effect of porcine and ovine FSH on nuclear maturation of pig oocytes in vitro

    Samartzi F, Tsakmakidis I, Theodosiadou E, Vainas E · Reproduction in domestic animals = Zuchthygiene · 2008PubMed Verified

  16. 16.
    Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production

    De Roover R, Feugang JM, Bols PE, Genicot G, Hanzen Ch · Reproduction in domestic animals = Zuchthygiene · 2008PubMed Verified

  17. 17.
    The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri-ovulatory period

    Leethongdee S, Khalid M, Bhatti A, Ponglowhapan S, Kershaw CM, Scaramuzzi RJ · Theriogenology · 2007PubMed Verified

  18. 18.
    Effects of progestagens on follicular growth and oocyte developmental competence in FSH-treated ewes

    Berlinguer F, Gonzalez-Bulnes A, Succu S, Leoni G, Mossa F, Bebbere D et al. · Domestic animal endocrinology · 2007PubMed Verified

  19. 19.
    The effect of melatonin implants during the seasonal anestrus on embryo production after superovulation in aged high-prolificacy Rasa Aragonesa ewes

    Forcada F, Abecia JA, Cebrián-Pérez JA, Muiño-Blanco T, Valares JA, Palacín I et al. · Theriogenology · 2006PubMed Verified

  20. 20.
    Plasma inhibin A determination at start superovulatory FSH treatments is predictive for embryo outcome in goats

    Gonzalez-Bulnes A, Garcia-Garcia RM, Carrizosa JA, Urrutia B, Souza CJ, Cocero MJ et al. · Domestic animal endocrinology · 2004PubMed Verified

  21. 21.
    Procedure for successful interspecific embryo transfer from mouflon (Ovis gmelini musimon) to Spanish Merino sheep (Ovis aries)

    Santiago-Moreno J, González-Bulnes A, Gómez-Brunet A, Cocero MJ, del Campo A, García-García R et al. · Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians · 2001PubMed Verified

  22. 22.
    Influence of maternal environment on the number of transferable embryos obtained in response to superovulatory FSH treatments in ewes

    González-Bulnes A, García-García RM, Castellanos V, Santiago-Moreno J, Ariznavarreta C, Domínguez V et al. · Reproduction, nutrition, development · 2003PubMed Verified

  23. 23.
    Reproductive season affects inhibitory effects from large follicles on the response to superovulatory FSH treatments in ewes

    Gonzalez-Bulnes A, Garcia-Garcia RM, Santiago-Moreno J, Dominguez V, Lopez-Sebastian A, Cocero MJ · Theriogenology · 2003PubMed Verified

  24. 24.
    Patterns of follicular growth in superovulated sheep and influence on endocrine and ovarian response

    Gonzalez-Bulnes A, Garcia-Garcia RM, Souza CJ, Santiago-Moreno J, Lopez-Sebastian A, Cocero MJ et al. · Reproduction in domestic animals = Zuchthygiene · 2002PubMed Verified

  25. 25.
    Effect of follicular status on superovulatory response in ewes is influenced by presence of corpus luteum at first FSH dose

    Gonzalez-Bulnes A, Garcia-Garcia RM, Santiago-Moreno J, Lopez-Sebastian A, Cocero MJ · Theriogenology · 2002PubMed Verified

  26. 26.
    Effects of oestradiol and progesterone on secretion of gonadotrophins and health of first wave follicles during the oestrous cycle of beef heifers

    Austin EJ, Mihm M, Evans AC, Ireland JL, Ireland JJ, Roche JF · Reproduction (Cambridge, England) · 2002PubMed Verified

  27. 27.
    Effects of FSH commercial preparation and follicular status on follicular growth and superovulatory response in Spanish Merino ewes

    Gonzalez-Bulnes A, Santiago-Moreno J, Cocero MJ, Lopez-Sebastian A · Theriogenology · 2000PubMed Verified

  28. 28.
  29. 29.
    Superovulatory response of dairy cattle (Bos taurus ) in a tropical environment

    Kanuya N, Callesen H, Hyttel P, Assey R, Greve T · Theriogenology · 1997PubMed Verified

  30. 30.
    Biopotency in vitro and metabolic clearance rates of five pituitary preparations of follicle stimulating hormone

    Phillips DJ, Hudson NL, Lun S, Condell LA, McNatty KP · Reproduction, fertility, and development · 1993PubMed Verified

  31. 31.
    Use of single or multiple injections of FSH in embryo collection programmes in goats

    Batt PA, Killeen ID, Cameron AW · Reproduction, fertility, and development · 1993PubMed Verified

  32. 32.
    Luteinizing hormone and follicle stimulating hormone induce premature condensation of chromatin in goat (Capra hircus) oocytes

    Kumar J, Osborn JC, Cameron AW · Reproduction, fertility, and development · 1991PubMed Verified

  33. 33.
    Oocyte production and ovarian steroid concentrations of immature rats in response to some commercial gonadotrophin preparations

    Henderson KM, Weaver A, Wards RL, Ball K, Lun S, Mullin C et al. · Reproduction, fertility, and development · 1990PubMed Verified

  34. 34.
    Superovulation and embryo recovery in goats treated with Ovagen and Folltropin

    McNatty KP, Hudson NL, Ball K, Mason A, Simmons MH · New Zealand veterinary journal · 1989PubMed Verified

Medical disclaimer: This information is for educational purposes only and does not constitute medical advice. Many compounds listed are research chemicals not approved for human use. Always consult a qualified healthcare professional before starting any protocol.

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