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AOD-9604

Also known as: Anti-Obesity Drug 9604, hGH Fragment 177-191, AOD9604

AOD-9604 is a modified fragment of human growth hormone (residues 177-191) with an additional tyrosine residue that significantly enhances bioavailability. Originally developed as an anti-obesity drug by Metabolic Pharmaceuticals, it stimulates lipolysis and inhibits lipogenesis without the diabetogenic effects of full GH.

Half-Life

30-45 minutes injectable; longer with nasal spray formulation

Route

SubQ, Intranasal, Oral

Category

Fat Loss & Metabolic

Studies

15 references

Key Benefits

  • Selective fat loss without anabolic side effects
  • No effect on blood glucose or insulin resistance
  • Improved bioavailability over Fragment 176-191
  • GRAS (Generally Recognized As Safe) status in Australia
  • Potential cartilage repair and anti-inflammatory properties
  • Does not suppress natural GH production

Mechanism of Action

AOD-9604 binds to a specific receptor on fat cells (distinct from IGF-1 receptor) to activate beta-3 adrenergic pathways, triggering fat breakdown. It inhibits lipogenesis and promotes lipolysis selectively in adipose tissue. Unlike Fragment 176-191, the added N-terminal tyrosine improves receptor binding affinity and oral bioavailability.

Dosing Protocols

Fat Loss Protocol

Dose
300-600 mcg
Frequency
Once daily
Timing
Upon waking on empty stomach
Cycle
12 weeks

Injectable subcutaneous or intranasal; morning dosing preferred for lipolytic effects

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Side Effects

  • Localized injection site reactions
  • Headache (rare)
  • Hypoglycemia risk in combination with insulin (very rare)

Storage

Lyophilized: store at -20°C for long term, 4°C for up to 4 weeks. Reconstituted: refrigerate at 4°C, use within 30 days.

  1. 1.
    Therapeutic Peptides in Orthopaedics: Applications, Challenges, and Future Directions

    Rahman OF, Lee SJ, Seeds WA · Journal of the American Academy of Orthopaedic Surgeons. Global research & reviews · 2026ReviewPubMed Verified

  2. 2.
    Simplifying and expanding the screening for peptides <2 kDa by direct urine injection, liquid chromatography, and ion mobility mass spectrometry

    Thomas A, Görgens C, Guddat S, Thieme D, Dellanna F, Schänzer W et al. · Journal of separation science · 2016PubMed Verified

  3. 3.
    Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid in Rabbit Osteoarthritis Model

    Kwon DR, Park GY · Annals of clinical and laboratory science · 2015PubMed Verified

  4. 4.
    Human sports drug testing by mass spectrometry

    Schänzer W, Thevis M · Mass spectrometry reviews · 2017ReviewPubMed Verified

  5. 5.
    Detecting peptidic drugs, drug candidates and analogs in sports doping: current status and future directions

    Thevis M, Thomas A, Schänzer W · Expert review of proteomics · 2014PubMed Verified

  6. 6.
    Detection and in vitro metabolism of AOD9604

    Cox HD, Smeal SJ, Hughes CM, Cox JE, Eichner D · Drug testing and analysis · 2015PubMed Verified

  7. 7.
  8. 8.
    Analytical approaches for the detection of emerging therapeutics and non-approved drugs in human doping controls

    Thevis M, Schänzer W · Journal of pharmaceutical and biomedical analysis · 2014ReviewPubMed Verified

  9. 9.
    AOD-9604 does not influence the WADA hGH isoform immunoassay

    Orlovius AK, Thomas A, Schänzer W, Thevis M · Drug testing and analysis · 2013PubMed Verified

  10. 10.
    Obesity drugs in clinical development

    Halford JC · Current opinion in investigational drugs (London, England : 2000) · 2006ReviewPubMed Verified

  11. 11.
    Gateways to clinical trials

    Bayés M, Rabasseda X, Prous JR · Methods and findings in experimental and clinical pharmacology · 2005PubMed Verified

  12. 12.
    AOD-9604 Metabolic

    Wilding J · Current opinion in investigational drugs (London, England : 2000) · 2004ReviewPubMed Verified

  13. 13.
    Gateways to clinical trials

    Bayes M, Rabasseda X, Prous JR · Methods and findings in experimental and clinical pharmacology · 2003PubMed Verified

  14. 14.
    Gateways to clinical trials

    Bayés M, Rabasseda X, Prous JR · Methods and findings in experimental and clinical pharmacology · 2003PubMed Verified

  15. 15.
    The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta(3)-AR knock-out mice

    Heffernan M, Summers RJ, Thorburn A, Ogru E, Gianello R, Jiang WJ et al. · Endocrinology · 2001PubMed Verified

Medical disclaimer: This information is for educational purposes only and does not constitute medical advice. Many compounds listed are research chemicals not approved for human use. Always consult a qualified healthcare professional before starting any protocol.

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