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5-Amino-1MQ

Also known as: 5-Amino-1-methylquinolinium, 5A1MQ

5-Amino-1MQ is a small-molecule NNMT (Nicotinamide N-methyltransferase) inhibitor that raises intracellular NAD+ levels and promotes fat burning. It is notable for targeting adipose tissue directly, reducing fat cell size and number while increasing metabolic rate.

Half-Life

Estimated 4–8 hours

Route

Oral

Category

Fat Loss & Metabolic

Studies

3 references

Key Benefits

  • Raises intracellular NAD+ levels
  • Directly targets adipose tissue for fat reduction
  • Reduces fat cell size and differentiation
  • Increases basal metabolic rate
  • SIRT1 activation for metabolic regulation
  • No stimulant cardiovascular side effects
  • Synergistic with intermittent fasting and caloric restriction
  • May have anti-aging metabolic benefits

Mechanism of Action

5-Amino-1MQ inhibits NNMT (Nicotinamide N-methyltransferase), an enzyme in adipose tissue that consumes SAM (S-adenosylmethionine) and depletes cellular NAD+. By blocking NNMT, it raises intracellular NAD+ levels in fat cells, activating SIRT1 and downstream pathways that promote fat oxidation. Studies show it reduces adipocyte differentiation, decreases fat cell lipid content, and increases resting metabolic rate without stimulant effects.

Dosing Protocols

Standard Fat Loss Protocol

Dose
50–100 mg
Frequency
Once to twice daily
Timing
With or without food
Cycle
8–12 weeks

Oral capsules. Well-tolerated in available research. May be stacked with other metabolic peptides/compounds. Limited long-term human data.

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Side Effects

  • Generally well-tolerated in available studies
  • Mild GI discomfort (rare)
  • Limited long-term human data

Contraindications

Pregnancy or breastfeeding. Limited human safety data.

Storage

Store oral capsules at room temperature away from moisture and heat.

  1. 1.
  2. 2.
    Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice

    Dimet-Wiley A, Wu Q, Wiley JT, Eswar A, Neelakantan H, Savidge T et al. · Scientific reports · 2022PubMed Verified

  3. 3.
    Small molecule inhibitor of nicotinamide N-methyltransferase shows anti-proliferative activity in HeLa cells

    Akar S, Duran T, Azzawri AA, Koçak N, Çelik Ç, Yıldırım Hİ · Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology · 2021PubMed Verified

Medical disclaimer: This information is for educational purposes only and does not constitute medical advice. Many compounds listed are research chemicals not approved for human use. Always consult a qualified healthcare professional before starting any protocol.

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